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  • 产品名称：anti-gamma-aminobutyricAcid(GABA)AReceptor,alpha1(GABRA1)(AA28-43)antibody

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  • 产品厂商：SYSY
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简单介绍：
anti-gamma-aminobutyricAcid(GABA)AReceptor,alpha1(GABRA1)(AA28-43)antibody
详情介绍：
Immunogen Synthetic peptide (aa 28-43 of rat GABA-A receptor alpha1 precursor protein) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
Specificity Specific for GABA-A receptor alpha1.
Purification Affinity purified with the immunogen. Guinea pig serum albumin was added for stabilization.
Alternative Name GABA-A Receptor Alpha1 (GABRA1 Antibody Abstract)
Background Synonyms: GABA(A) R alpha1
Application Notes WB: 1 : 1000 up to 1 : 2000 (AP staining)
IP: not tested yet
ICC: 1 : 200 up to 1 : 1000
IHC: 1 : 500
Comment

WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ICC: This antibody is PFA fixation sensitive, use only mild fixation (2% PFA). Best results are obtained by application on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents. IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008).
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Western Blotting (WB) image for anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1) (AA 28-43) antibody (ABIN1742457) dilution: 1 : 1000, sample: unboiled synaptic membrane fraction of rat brain (LP1)
Immunocytochemistry (ICC) image for anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1) (AA 28-43) antibody (ABIN1742457) Indirect immunostaining of hippocampus neurons with anti-GABA-A receptor α1 (dilution...
Immunohistochemistry (IHC) image for anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1) (AA 28-43) antibody (ABIN1742457) Indirect immunostaining of PFA fixed mouse brain sections (dilution 1 : 500).