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  • 产品名称：anti-Synaptotagmin1/2(SYT1/2)(AA120-131),(Cytoplasmic)antibody

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  • 产品厂商：SYSY
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简单介绍：
anti-Synaptotagmin1/2(SYT1/2)(AA120-131),(Cytoplasmic)antibody
详情介绍：
Immunogen Synthetic peptide DDDAETGLTDGE (aa 120-131 in rat synaptotagmin 1) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Cross-Reactivity (Details) Some cross-reactivity to synaptotagmin 2.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Alternative Name Synaptotagmin 1, 2
Background Synonyms: p65
Application Notes WB: 1 : 1000 (AP staining)
ICC: 1 : 500 up to 1 : 1000
IHC: 1 : 200 up to 1 : 500
IHC-P: not tested yet
Comment

ELISA: Suitable as detector antibody for sandwich-ELISA as capture antibodies (protocol for sandwich-ELISA).
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μl H2O to get a 1mg/ml solution of antibody in PBS, then aliquot and store at -20°C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Western Blotting (WB) image for anti-Synaptotagmin 1/2 (SYT1/2) (AA 120-131), (Cytoplasmic) antibody (ABIN1742197) dilution: 1 : 1000, sample: crude synaptosomal fraction of rat brain (P2)
Immunocytochemistry (ICC) image for anti-Synaptotagmin 1/2 (SYT1/2) (AA 120-131), (Cytoplasmic) antibody (ABIN1742197) Immunofluorescence labeling of PFA fixed cultured hippocampus neurons (dilution 1 : 500)
Immunohistochemistry (IHC) image for anti-Synaptotagmin 1/2 (SYT1/2) (AA 120-131), (Cytoplasmic) antibody (ABIN1742197) Indirect immunolabeling of PFA fixed mouse hippocampus section (dilution 1 : 200). Nu...