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  • 产品名称：anti-GFPantibody(GreenFluorescentProtein)(CypHer5E)

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  • 产品厂商：SYSY
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简单介绍：
anti-GFPantibody(GreenFluorescentProtein)(CypHer5E)
详情介绍：
Immunogen Strep-Tag? fusion protein, full-length GFP.
Cross-Reactivity (Details) Cross reacts with all GFP derivatives tested so far (e.g. EYFP, ECFP, EGFP).
Purification Affinity purified with the immunogen, fluorescence-labeled with CypHer5E. Rabbit serum albumin was added for stabilization.
Alternative Name GFP
Research Area Tags/Labels
Application Notes IP: not tested yet
ICC: 1 : 100 up to 1 : 1000
IHC-P: not tested yet
Comment

ABIN1742297 Suitable for internalization studies when GFP is fused to an epitope residing in the lumen of an acidic compartment.
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol (if this does not interfere with your experiments!), then aliquot and store at -20 °C until use, or store aliquots at -80 °C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Fluorescence-labeled antibodies should be reconstituted immediately upon delivery.
Prolonged storage at 4 °C is not recommended!
Product cited in: Tatavarty, Sun, Turrigiano: "How to scale down postsynaptic strength." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 32, pp. 13179-89, 2013 (PubMed).