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  • 产品名称:anti-VacuolarProteinSorting45Homolog(S.Cerevisiae)(VPS45)antibody

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简单介绍:
anti-VacuolarProteinSorting45Homolog(S.Cerevisiae)(VPS45)antibody
详情介绍:
Immunogen Recombinant full-length human VPS 45.
Specificity Specific for VPS 45.
Purification antiserum
Alternative Name VPS 45 (VPS45 Antibody Abstract)
Pathways Transition Metal Ion Homeostasis, Synaptic Vesicle Exocytosis
Application Notes WB: 1 : 100 up to 1 : 5000 (AP staining)
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
Restrictions For Research Use only
Format Liquid
Buffer PBS
Handling Advice Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4?°C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20?°C),however, is preferable.
Storage 4 °C/-20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-Vacuolar Protein Sorting 45 Homolog (S. Cerevisiae) (VPS45) antibody (ABIN1742303) dilution: 1 : 1000, sample: crude synaptosomal fraction of rat brain (P2)
Product cited in: Roccisana, Sadler, Bryant, Gould: "Sorting of GLUT4 into its insulin-sensitive store requires the Sec1/Munc18 protein mVps45." in: Molecular biology of the cell, Vol. 24, Issue 15, pp. 2389-97, 2013 (PubMed).

Waugh, Chu, Clayton, Minogue, Hsuan: "Detergent-free isolation and characterization of cholesterol-rich membrane domains from trans-Golgi network vesicles." in: Journal of lipid research, Vol. 52, Issue 3, pp. 582-9, 2011 (PubMed).

Rahajeng, Caplan, Naslavsky: "Common and distinct roles for the binding partners Rabenosyn-5 and Vps45 in the regulation of endocytic trafficking in mammalian cells." in: Experimental cell research, Vol. 316, Issue 5, pp. 859-74, 2010 (PubMed).

Takamori, Holt, Stenius, Lemke, Gr?nborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gr?ter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, 2006 (PubMed).

Larance, Ramm, St?ckli, van Dam, Winata, Wasinger, Simpson, Graham, Junutula, Guilhaus, James: "Characterization of the role of the Rab GTPase-activating protein AS160 in insulin-regulated GLUT4 trafficking." in: The Journal of biological chemistry, Vol. 280, Issue 45, pp. 37803-13, 2005 (PubMed).