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  • 产品名称：anti-SynaptotagminII(SYT2)(AA406-422),(CytoplasmicDomain)antibody

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  • 产品厂商：SYSY
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简单介绍：
anti-SynaptotagminII(SYT2)(AA406-422),(CytoplasmicDomain)antibody
详情介绍：
Immunogen Synthetic peptide (aa 406-422 in rat synaptotagmin 2) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Specificity Specific for synaptotagmin 2.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Alternative Name Synaptotagmin 2 (SYT2 Antibody Abstract)
Pathways Synaptic Vesicle Exocytosis
Application Notes WB: 1 : 1000 (AP staining)
ICC: not tested yet
IHC: 1 : 500 up to 1 : 1000
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Western Blotting (WB) image for anti-Synaptotagmin II (SYT2) (AA 406-422), (Cytoplasmic Domain) antibody (ABIN1742202) dilution: 1 : 1000, sample: synaptic vesicle fraction of rat brain (LP2)
Immunohistochemistry (IHC) image for anti-Synaptotagmin II (SYT2) (AA 406-422), (Cytoplasmic Domain) antibody (ABIN1742202) anti-Synaptotagmin II (SYT2) (AA 406-422), (Cytoplasmic Domain) antibody (Image 2)
Product cited in: Whitemarsh, Strathman, Chase, Stankewicz, Tepp, Johnson, Pellett: "Novel application of human neurons derived from induced pluripotent stem cells for highly sensitive botulinum neurotoxin detection." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 126, Issue 2, pp. 426-35, 2012 (PubMed).

Culver, Savas, Park, Choi, Zheng, Zeitlin, Yates, Tanese: "Proteomic analysis of wild-type and mutant huntingtin-associated proteins in mouse brains identifies unique interactions and involvement in protein synthesis." in: The Journal of biological chemistry, Vol. 287, Issue 26, pp. 21599-614, 2012 (PubMed).

Johnson, Franz, Kuhn, Furness, Rüttiger, Münkner, Rivolta, Seward, Herschman, Engel, Knipper, Marcotti: "Synaptotagmin IV determines the linear Ca2+ dependence of vesicle fusion at auditory ribbon synapses." in: Nature neuroscience, Vol. 13, Issue 1, pp. 45-52, 2009 (PubMed).