产品详情
所在位置: 首页> 产品目录> 一抗>
  • 产品名称:anti-SNAP25antibody(Synaptosomal-AssociatedProtein,25kDa)(AA192-206)

  • 产品型号:
  • 产品厂商:SYSY
  • 产品文档:
你添加了1件商品 查看购物车
简单介绍:
anti-SNAP25antibody(Synaptosomal-AssociatedProtein,25kDa)(AA192-206)
详情介绍:
Immunogen Synthetic peptide IDEANQRATKMLGSG (aa 192-206 in human SNAP 25) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Specificity Specific for SNAP 25.
Purification antiserum
Alternative Name SNAP 25 (SNAP25 Antibody Abstract)
Pathways Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
Application Notes WB: 1 : 10000 (AP staining)
ICC: 1 : 100
Comment

Recognizes the Botulinum neurotoxin A cleavage product with reduced affinity. Does not detect the neurotoxin E cleavage product. Recognizes splice variants SNAP 25A and B. ELISA: Suitable as detector antibody for sandwich-ELISA as capture antibody (protocol for sandwich-ELISA).

Restrictions For Research Use only
Format Liquid
Buffer PBS
Handling Advice Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4?°C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20?°C),however, is preferable.
Storage 4 °C/-20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) (AA 192-206) antibody (ABIN1742235) dilution: 1 : 1000, sample: crude synaptosomal fraction of rat brain (P2)
Immunohistochemistry (IHC) image for anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) (AA 192-206) antibody (ABIN1742235) Indirect immunostaining of a PFA fixed mouse hippocampus section with anti-SNAP 25 (d...
Immunocytochemistry (ICC) image for anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) (AA 192-206) antibody (ABIN1742235) Indirect immunostaining of PFA fixed rat hippocampus neurons (dilution 1 : 500; green...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) (AA 192-206) antibody (ABIN1742235) Indirect immunofluorescence labeling of PFA fixed, paraffin embedded mouse cerebellum...
Product cited in: Schafer, Heller, Gunner, Heller, Gordon, Hammond, Wolf, Jung, Stevens: "Microglia contribute to circuit defects in Mecp2 null mice independent of microglia-specific loss of Mecp2 expression." in: eLife, Vol. 5, 2016 (PubMed).

Fossati, Morini, Corradini, Antonucci, Trepte, Edry, Sharma, Papale, Pozzi, Defilippi, Meier, Brambilla, Turco, Rosenblum, Wanker, Ziv, Menna, Matteoli: "Reduced SNAP-25 increases PSD-95 mobility and impairs spine morphogenesis." in: Cell death and differentiation, Vol. 22, Issue 9, pp. 1425-36, 2015 (PubMed).

Walter, Kurps, de Wit, Sch?ning, Toft-Bertelsen, Lauks, Ziomkiewicz, Weiss, Schulz, Fischer von Mollard, Verhage, S?rensen: "The SNARE protein vti1a functions in dense-core vesicle biogenesis." in: The EMBO journal, Vol. 33, Issue 15, pp. 1681-97, 2014 (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kr?hnert, Sch?fer, Rammner, Koo, Cla?en, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, 2014 (PubMed).

Kung, Gong, Adedoyin, Ng, Bhargava, Ohara, Jasmin: "Evidence for glutamate as a neuroglial transmitter within sensory ganglia." in: PLoS ONE, Vol. 8, Issue 7, pp. e68312, 2013 (PubMed).

Young, Franciosi, Spreeuw, Deng, Sanders, Tam, Huang, Singaraja, Zhang, Bissada, Kay, Hayden: "Low levels of human HIP14 are sufficient to rescue neuropathological, behavioural, and enzymatic defects due to loss of murine HIP14 in Hip14-/- mice." in: PLoS ONE, Vol. 7, Issue 5, pp. e36315, 2012 (PubMed).

Vasileva, Horstmann, Geumann, Gitler, Kuner: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." in: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, 2012 (PubMed).

Camoletto, Vara, Morando, Connell, Marletto, Giustetto, Sassoè-Pognetto, Van Veldhoven, Ledesma: "Synaptic vesicle docking: sphingosine regulates syntaxin1 interaction with Munc18." in: PLoS ONE, Vol. 4, Issue 4, pp. e5310, 2009 (PubMed).

Johansson, Ericsson, Janson, Beraki, Stani?, Mandic, Wikstr?m, H?kfelt, Ogren, Rozell, Berggren, Bark: "An ancient duplication of exon 5 in the Snap25 gene is required for complex neuronal development/function." in: PLoS genetics, Vol. 4, Issue 11, pp. e1000278, 2008 (PubMed).