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  • 产品名称：anti-Syntaxin12/13(AA1-250),(CytoplasmicDomain)antibody

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  • 产品厂商：SYSY
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简单介绍：
anti-Syntaxin12/13(AA1-250),(CytoplasmicDomain)antibody
详情介绍：
Immunogen Recombinant cytoplasmic domain of rat syntaxin 13 (aa 1-250).
Clone 151-2
Isotype IgG1
Specificity Specific for syntaxin 12/13.
Purification purified IgG
Application Notes WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 100 up to 1 : 500
IHC: not tested yet
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 100 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1?mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Storage -20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-Syntaxin 12/13 (AA 1-250), (Cytoplasmic Domain) antibody (ABIN1742228) dilution: 1 : 1000, sample: crude synaptosomal fraction of rat brain (P2)
Immunocytochemistry (ICC) image for anti-Syntaxin 12/13 (AA 1-250), (Cytoplasmic Domain) antibody (ABIN1742228) Indirect immunofluorescence on cultured rat neurons (dilution 1 : 250; red). Nuclei a...
Product cited in: Widagdo, Chai, Ridder, Chau, Johnson, Sah, Huganir, Anggono: "Activity-Dependent Ubiquitination of GluA1 and GluA2 Regulates AMPA Receptor Intracellular Sorting and Degradation." in: Cell reports, 2015 (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kr?hnert, Sch?fer, Rammner, Koo, Cla?en, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, 2014 (PubMed).

Deane, Ilie, Sizdahkhani, Das Gupta, Orlowski, McKinney: "Enhanced recruitment of endosomal Na+/H+ exchanger NHE6 into Dendritic spines of hippocampal pyramidal neurons during NMDA receptor-dependent long-term potentiation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 2, pp. 595-610, 2013 (PubMed).