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  • 产品名称：anti-GABRA3antibody(gamma-aminobutyricAcid(GABA)AReceptor,alpha3)(AA29-43)

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  • 产品厂商：SYSY
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简单介绍：
anti-GABRA3antibody(gamma-aminobutyricAcid(GABA)AReceptor,alpha3)(AA29-43)
详情介绍：
Immunogen Synthetic peptide (aa 29-43 of rat GABA-A receptor alpha3 precursor protein) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
Specificity Specific for GABA-A receptor alpha3.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Alternative Name GABA-A Receptor Alpha3 (GABRA3 Antibody Abstract)
Background Synonyms: GABA(A) R alpha3
Application Notes WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 500
Comment

WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008).
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Western Blotting (WB) image for anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3) (AA 29-43) antibody (ABIN1742459) dilution: 1 : 1000, sample: unboiled synaptic membrane fraction of rat brain (LP1)
Immunocytochemistry (ICC) image for anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3) (AA 29-43) antibody (ABIN1742459) Indirect immunostaining of hippocampus neurons with anti-GABA-A receptor α3 (dilution...
Immunohistochemistry (IHC) image for anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3) (AA 29-43) antibody (ABIN1742459) Indirect immunostaining of PFA fixed mouse brain sections (dilution 1 : 500).
Product cited in: Tzanoulinou, García-Mompó, Castillo-Gómez, Veenit, Nacher, Sandi: "Long-term behavioral programming induced by peripuberty stress in rats is accompanied by GABAergic-related alterations in the Amygdala." in: PLoS ONE, Vol. 9, Issue 4, pp. e94666, 2014 (PubMed).