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  • 产品名称：anti-VAMP1,2,3(AA1-81)antibody

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  • 产品厂商：SYSY
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简单介绍：
anti-VAMP1,2,3(AA1-81)antibody
详情介绍：
Immunogen Recombinant protein of the cytoplasmic part of rat cellubrevin (aa 1-81).
Specificity Specific for all three isoforms VAMP 1, 2 and 3.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Background Synonyms: VAMP 3
Application Notes WB: 1 : 1000 up to 1 : 5000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 200
Comment

ICC: Methanol and acetone fixation, see Annaert et al., 1997 This antibody is recommended for ICC of non-neuronal cells. This antibody detects the tetanus and Botulinum B toxin cleavage product in toxin treated tissue homogenates.
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Immunocytochemistry (ICC) image for anti-VAMP1, 2, 3 (AA 1-81) antibody (ABIN1742193) Immunofluorescence labeling of hippocampus neurons, (dilution 1 : 500).
Western Blotting (WB) image for anti-VAMP1, 2, 3 (AA 1-81) antibody (ABIN1742193) dilution: 1 : 1000, sample: crude synaptosomal fraction of rat brain (P2)
Immunohistochemistry (IHC) image for anti-VAMP1, 2, 3 (AA 1-81) antibody (ABIN1742193) Indirect immunostaining of a PFA fixed mouse hippocampus section (dilution 1 : 200).
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-VAMP1, 2, 3 (AA 1-81) antibody (ABIN1742193) Indirect immunostaining of PFA fixed paraffin embedded mouse hippocampus section (dil...
Product cited in: Arsenault, Cuijpers, Ferrari, Niranjan, Rust, Leese, OBrien, Binz, Davletov: "Botulinum protease-cleaved SNARE fragments induce cytotoxicity in neuroblastoma cells." in: Journal of neurochemistry, Vol. 129, Issue 5, pp. 781-91, 2014 (PubMed).