anti-gamma-aminobutyricAcid(GABA)AReceptor,Subunitbeta1(GABRB1)(AA342-430)antib 抗体网

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产品名称：anti-gamma-aminobutyricAcid(GABA)AReceptor,Subunitbeta1(GABRB1)(AA342-430)antib
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产品厂商：SYSY
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简单介绍：

anti-gamma-aminobutyricAcid(GABA)AReceptor,Subunitbeta1(GABRB1)(AA342-430)antibody

详情介绍：

Product details
Product details
Immunogen	Recombinant protein (aa 342-430 in mouse) of the unprocessed precursor forced into a loop-shaped structure.
Specificity	Specific for GABA-A receptor beta1.
Purification	Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Target details
Target details
Alternative Name	GABA-A Receptor Beta1 (GABRB1 Antibody Abstract)
Background	Synonyms: GABA(A) R beta1

Application Details
Application Details
Application Notes	WB: 1 : 1000 (AP staining) IP: not tested yet ICC: 1 : 500 IHC: 1 : 500
Comment	WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008).
Restrictions	For Research Use only

Handling
Handling
Format	Lyophilized
Reconstitution	For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer	PBS
Handling Advice	Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage	-20 °C
Storage Comment	Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Images
Images
Supplier Images	dilution: 1 : 1000, sample: unboiled synaptic membrane fraction of rat brain (LP1) Indirect immunostaining of PFA fixed mouse cortex neurons with anti-GABA-A receptor β... Indirect immunostaining of a rat hippocampus section fixed with 4 % PFA and 15 % picr... Images for product: anti-gamma-aminobutyric Acid (GABA) A Receptor, Subunit beta 1 (GABRB1) (AA 342-430) antibody dilution: 1 : 1000, sample: unboiled synaptic membrane fraction of rat brain (LP1) Indirect immunostaining of PFA fixed mouse cortex neurons with anti-GABA-A receptor β1 (dilution 1 : 500; red) and anti-synapsin (cat. no. 106 001, dilution 1 : 500; green). Indirect immunostaining of a rat hippocampus section fixed with 4 % PFA and 15 % picric acid (dilution 1 : 500).

References
References
Product cited in:	Kerti-Szigeti, Nusser: "Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells." in: eLife, Vol. 5, 2016 (PubMed).