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  • 产品名称:anti-RegulatingSynapticMembraneExocytosis2(RIMS2)(AA461-987)antibody

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简单介绍:
anti-RegulatingSynapticMembraneExocytosis2(RIMS2)(AA461-987)antibody
详情介绍:
Immunogen Recombinant protein (aa 461-987) of rat RIM 2 containing part of PDZ domain.
Specificity RIM 2 including splice variants
Cross-Reactivity (Details) cross reactivity to RIM 1.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Alternative Name RIM 2 (RIMS2 Antibody Abstract)
Pathways Hormone Transport, Synaptic Vesicle Exocytosis
Application Notes WB: 1 : 100 up to 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 200 up to 1 : 500
IHC: 1 : 1000
Comment

This antibody works with paraformaldehyde and methanol fixation.

Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Western Blotting (WB) image for anti-Regulating Synaptic Membrane Exocytosis 2 (RIMS2) (AA 461-987) antibody (ABIN1742308) dilution: 1 : 100, sample: crude synaptic membranes fraction of rat brain (LP1)
Immunohistochemistry (IHC) image for anti-Regulating Synaptic Membrane Exocytosis 2 (RIMS2) (AA 461-987) antibody (ABIN1742308) Indirect immunostaining of ribbon synapses in the outer plexiform layer of PFA mouse ...
Product cited in: Mendoza Schulz, Jing, Sánchez Caro, Wetzel, Dresbach, Strenzke, Wichmann, Moser: "Bassoon-disruption slows vesicle replenishment and induces homeostatic plasticity at a CNS synapse." in: The EMBO journal, Vol. 33, Issue 5, pp. 512-27, 2014 (PubMed).

de Andrade, Kunzelman, Merrill, Fuerst: "Developmentally dynamic colocalization patterns of DSCAM with adhesion and synaptic proteins in the mouse retina." in: Molecular vision, Vol. 20, pp. 1422-33, 2014 (PubMed).

Gardezi, Li, Stanley: "Inter-channel scaffolding of presynaptic CaV2.2 via the C terminal PDZ ligand domain." in: Biology open, Vol. 2, Issue 5, pp. 492-8, 2013 (PubMed).

Limbach, Laue, Wang, Hu, Thiede, Hultqvist, Kilimann: "Molecular in situ topology of Aczonin/Piccolo and associated proteins at the mammalian neurotransmitter release site." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 31, pp. E392-401, 2011 (PubMed).

Gebhart, Juhasz-Vedres, Zuccotti, Brandt, Engel, Trockenbacher, Kaur, Obermair, Knipper, Koschak, Striessnig: "Modulation of Cav1.3 Ca2+ channel gating by Rab3 interacting molecule." in: Molecular and cellular neurosciences, Vol. 44, Issue 3, pp. 246-59, 2010 (PubMed).

Uthaiah, Hudspeth: "Molecular anatomy of the hair cell's ribbon synapse." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 37, pp. 12387-99, 2010 (PubMed).

Regus-Leidig, Tom Dieck, Specht, Meyer, Brandst?tter: "Early steps in the assembly of photoreceptor ribbon synapses in the mouse retina: the involvement of precursor spheres." in: The Journal of comparative neurology, Vol. 512, Issue 6, pp. 814-24, 2008 (PubMed).

Kwan, Xie, Sheu, Ohtsuka, Gaisano: "Interaction between Munc13-1 and RIM is critical for glucagon-like peptide-1 mediated rescue of exocytotic defects in Munc13-1 deficient pancreatic beta-cells." in: Diabetes, Vol. 56, Issue 10, pp. 2579-88, 2007 (PubMed).

Khanna, Zougman, Stanley: "A proteomic screen for presynaptic terminal N-type calcium channel (CaV2.2) binding partners." in: Journal of biochemistry and molecular biology, Vol. 40, Issue 3, pp. 302-14, 2007 (PubMed).

Inoue, Deguchi-Tawarada, Takao-Rikitsu, Inoue, Kitajima, Ohtsuka, Takai: "ELKS, a protein structurally related to the active zone protein CAST, is involved in Ca2+-dependent exocytosis from PC12 cells." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 11, Issue 6, pp. 659-72, 2006 (PubMed).

Juranek, Mukherjee, Rickmann, Martens, Calka, Südhof, Jahn: "Differential expression of active zone proteins in neuromuscular junctions suggests functional diversification." in: The European journal of neuroscience, Vol. 24, Issue 11, pp. 3043-52, 2006 (PubMed).

tom Dieck, Altrock, Kessels, Qualmann, Regus, Brauner, Fejtová, Bracko, Gundelfinger, Brandst?tter: "Molecular dissection of the photoreceptor ribbon synapse: physical interaction of Bassoon and RIBEYE is essential for the assembly of the ribbon complex." in: The Journal of cell biology, Vol. 168, Issue 5, pp. 825-36, 2005 (PubMed).

Ohara-Imaizumi, Ohtsuka, Matsushima, Akimoto, Nishiwaki, Nakamichi, Kikuta, Nagai, Kawakami, Watanabe, Nagamatsu et al.: "ELKS, a protein structurally related to the active zone-associated protein CAST, is expressed in pancreatic beta cells and functions in insulin exocytosis: interaction of ELKS with exocytotic ..." in: Molecular biology of the cell, Vol. 16, Issue 7, pp. 3289-300, 2005 (PubMed).