anti-Piccolo(PIC)(AA2011-2350)antibody 抗体网

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产品名称：anti-Piccolo(PIC)(AA2011-2350)antibody
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anti-Piccolo(PIC)(AA2011-2350)antibody

详情介绍：

Product details
Product details
Immunogen	Recombinant rat piccolo (aa 2011-2350).
Specificity	Specific for piccolo.
Purification	antiserum

Target details
Target details
Alternative Name	Piccolo
Background	Synonyms: Aczonin
Research Area	Neurology

Application Details
Application Details
Application Notes	WB: 1 : 1000 (AP staining) IP: not tested yet ICC: 1 : 500 up to 1 : 1000 IHC: 1 : 200
Comment	WB: Due to its large size, piccolo requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed. This antibody detects an additional band of ~65 kDa.
Restrictions	For Research Use only

Handling
Handling
Format	Liquid
Buffer	PBS
Handling Advice	Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4?°C. Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20?°C),however, is preferable.
Storage	4 °C/-20 °C
Storage Comment	Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Images
Images
Supplier Images	dilution: 1 : 1000, sample: mouse brain homogenate Indirect immunostaining of rat hippocampus neurons (dilution 1 : 500). Indirect immunofluorescence labeling of a PFA fixed mouse cerebellum section with ant... Indirect immunostaining of PFA fixed paraffin embedded mouse hippocampus section with... Images for product: anti-Piccolo (PIC) (AA 2011-2350) antibody dilution: 1 : 1000, sample: mouse brain homogenate Indirect immunostaining of rat hippocampus neurons (dilution 1 : 500). Indirect immunofluorescence labeling of a PFA fixed mouse cerebellum section with anti-piccolo (dilution 1 : 200; red) and rabbit anti-Sec22b (cat. no. 186 003, dilution 1 : 200; green). Nuclei have been visualized by DAPI staining (blue). Indirect immunostaining of PFA fixed paraffin embedded mouse hippocampus section with anti-Piccolo (dilution 1 : 500; green) and rabbit anti-Homer 1 (cat. no. 160 003, dilution 1 : 500; red). Nuclei have been visualized by DAPI staining (blue).

References
References
Product cited in:	Babai, Sendelbeck, Regus-Leidig, Fuchs, Mertins, Reim, Brose, Feigenspan, Brandst?tter: "Functional Roles of Complexin3 and Complexin4 at Mouse Photoreceptor Ribbon Synapses." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 36, Issue 25, pp. 6651-67, 2016 (PubMed).