产品详情
所在位置: 首页> 产品目录> 一抗>
查看大图

  • 产品名称：anti-Unc-13HomologA(C.Elegans)(UNC13A)(AA3-317)antibody

  • 产品型号：
  • 产品厂商：SYSY
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
anti-Unc-13HomologA(C.Elegans)(UNC13A)(AA3-317)antibody
详情介绍：
Immunogen Recombinant rat munc 13-1 (aa 3-317).
Clone 266B1
Isotype IgG2b
Specificity Specific for munc 13-1.
Purification purified IgG
Alternative Name Munc 13-1 (UNC13A Antibody Abstract)
Pathways Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis
Application Notes WB: 1 : 500 up to 1 : 5000 (AP staining)
ICC: limited
IHC: not tested yet
Comment

IP: For most effective IP use the solubilization protocol described in the ELISA protocol. Consider that protein-protein interaction may be affected. ICC: limited to methanol fixation. ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 100 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1?mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Storage -20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-Unc-13 Homolog A (C. Elegans) (UNC13A) (AA 3-317) antibody (ABIN1742276) dilution: 1 : 500, sample: crude synaptosomal fraction of rat brain (P2)
Product cited in: Kaeser-Woo, Younts, Yang, Zhou, Wu, Castillo, Südhof: "Synaptotagmin-12 phosphorylation by cAMP-dependent protein kinase is essential for hippocampal mossy fiber LTP." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 23, pp. 9769-80, 2013 (PubMed).

Dudenh?ffer-Pfeifer, Schirra, Pattu, Halimani, Maier-Peuschel, Marshall, Matti, Becherer, Dirks, Jung, Lipp, Hoth, Sester, Krause, Rettig: "Different Munc13 isoforms function as priming factors in lytic granule release from murine cytotoxic T lymphocytes." in: Traffic (Copenhagen, Denmark), Vol. 14, Issue 7, pp. 798-809, 2013 (PubMed).

Maas, Torres, Altrock, Leal-Ortiz, Wagh, Terry-Lorenzo, Fejtova, Gundelfinger, Ziv, Garner: "Formation of Golgi-derived active zone precursor vesicles." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 32, pp. 11095-108, 2012 (PubMed).

Martín, Bartolomé-Martín, Torres, Sánchez-Prieto: "Non-additive potentiation of glutamate release by phorbol esters and metabotropic mGlu7 receptor in cerebrocortical nerve terminals." in: Journal of neurochemistry, Vol. 116, Issue 4, pp. 476-85, 2011 (PubMed).

Martín, Durroux, Ciruela, Torres, Pin, Sánchez-Prieto: "The metabotropic glutamate receptor mGlu7 activates phospholipase C, translocates munc-13-1 protein, and potentiates glutamate release at cerebrocortical nerve terminals." in: The Journal of biological chemistry, Vol. 285, Issue 23, pp. 17907-17, 2010 (PubMed).

Takamori, Holt, Stenius, Lemke, Gr?nborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gr?ter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, 2006 (PubMed).