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  • 产品名称：anti-NSFantibody(N-Ethylmaleimide-SensitiveFactor)

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  • 产品厂商：SYSY
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简单介绍：
anti-NSFantibody(N-Ethylmaleimide-SensitiveFactor)
详情介绍：
Immunogen Recombinant full length rat NSF.
Clone 83-11
Isotype IgG1
Specificity Specific for NSF.
Purification purified IgG
Alternative Name NSF (NSF Antibody Abstract)
Research Area Signaling, Metabolism
Application Notes WB: 1 : 1000 (AP staining)
ICC: 1 : 500
IHC-P: 1 : 1000
Comment

ELISA: Suitable as capture antibody for sandwich-ELISA with ABIN1742270 as detector antibody (protocol for sandwich-ELISA).
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 100 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1?mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Storage -20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-N-Ethylmaleimide-Sensitive Factor (NSF) antibody (ABIN1742269) dilution: 1 : 1000, sample: crude synaptosomal fraction of rat brain (P2)
Immunocytochemistry (ICC) image for anti-N-Ethylmaleimide-Sensitive Factor (NSF) antibody (ABIN1742269) Indirect immunostaining of hippocampus neurons (dilution 1 : 500)
Immunohistochemistry (IHC) image for anti-N-Ethylmaleimide-Sensitive Factor (NSF) antibody (ABIN1742269) anti-N-Ethylmaleimide-Sensitive Factor (NSF) antibody (Image 3)
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-N-Ethylmaleimide-Sensitive Factor (NSF) antibody (ABIN1742269) Immunostaining of paraffin embedded sections from mouse hippocampus (dilution 1 : 100...
Product cited in: Hoopmann, Punge, Barysch, Westphal, Bückers, Opazo, Bethani, Lauterbach, Hell, Rizzoli: "Endosomal sorting of readily releasable synaptic vesicles." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 44, pp. 19055-60, 2010 (PubMed).

Ohno, Ishihara, Terada, Kikuta, Sofue, Kawai, Serikawa, Sasa: "Preferential increase in the hippocampal synaptic vesicle protein 2A (SV2A) by pentylenetetrazole kindling." in: Biochemical and biophysical research communications, Vol. 390, Issue 3, pp. 415-20, 2009 (PubMed).