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  • 产品名称：anti-SYT1antibody(SynaptotagminI)(AA1-8)

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  • 产品厂商：SYSY
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简单介绍：
anti-SYT1antibody(SynaptotagminI)(AA1-8)
详情介绍：
Immunogen Synthetic peptide MVSASRPE (aa 1-8 in mouse synaptotagmin 1) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
Specificity Specific for synaptotagmin 1
Cross-Reactivity (Details) no cross-reactivity to synaptotagmin 2.
Purification antiserum
Alternative Name Synaptotagmin 1 (SYT1 Antibody Abstract)
Background Synonyms: p65
Research Area Neurology
Pathways Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
Application Notes WB: 1 : 2000 (AP staining)
IP: not tested yet
ICC: 1 : 500 up to 1 : 5000
IHC: not tested yet
Comment

This antibody can be used for labeling of recycling synaptic vesicles by adding to living neurons or as a marker for exocytosis in isolated nerve terminals.
Restrictions For Research Use only
Format Liquid
Buffer PBS
Handling Advice Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4?°C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20?°C),however, is preferable.
Storage 4 °C/-20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-Synaptotagmin I (SYT1) (AA 1-8), (Lumenal Region) antibody (ABIN1742200) dilution: 1 : 1000, sample: crude synaptosomal fraction of rat brain (P2)
Immunocytochemistry (ICC) image for anti-Synaptotagmin I (SYT1) (AA 1-8), (Lumenal Region) antibody (ABIN1742200) Labeling of recycling vesicles in cultured rat hippocampus neurons (dilution 1 : 1000...
Product cited in: Shinoda, Ahmed, Ramachandran, Bharat, Brockelt, Altas, Dean: "BDNF enhances spontaneous and activity-dependent neurotransmitter release at excitatory terminals but not at inhibitory terminals in hippocampal neurons." in: Frontiers in synaptic neuroscience, Vol. 6, pp. 27, 2014 (PubMed).