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  • 产品名称：anti-HighAffinityCholineTransporter(ChT)(AA501-580)antibody

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简单介绍：
anti-HighAffinityCholineTransporter(ChT)(AA501-580)antibody
详情介绍：
Immunogen Recombinant protein of rat ChT (aa 501-580).
Clone 62-2E8
Isotype IgG1
Specificity Specific for the high affinity choline transporter.
Purification purified IgG
Alternative Name ChT (ChT Antibody Abstract)
Background Synonyms: Choline transporter, SLC5A7
Application Notes WB: 1 : 1000 (AP staining)
IHC: 1 : 100
Comment

ChT aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 100 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1?mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Storage -20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-High Affinity Choline Transporter (ChT) (AA 501-580) antibody (ABIN1742430) dilution: 1 : 1000, sample: enriched vesicles from rat spinal chord
Immunohistochemistry (IHC) image for anti-High Affinity Choline Transporter (ChT) (AA 501-580) antibody (ABIN1742430) A: Indirect immunohystochemistry of a mouse striatum section (dilution 1 : 100). B: I...
Product cited in: Misawa, Fujigaya, Nishimura, Moriwaki, Okuda, Kawashima, Nakata, Ruggiero, Blakely, Nakatsu, Ohno: "Aberrant trafficking of the high-affinity choline transporter in AP-3-deficient mice." in: The European journal of neuroscience, Vol. 27, Issue 12, pp. 3109-17, 2008 (PubMed).