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  • 产品名称：anti-GAD65antibody(GlutamateDecarboxylase2(PancreaticIsletsandBrain,65kDa))(AA3

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简单介绍：
anti-GAD65antibody(GlutamateDecarboxylase2(PancreaticIsletsandBrain,65kDa))(AA3-96)
详情介绍：
Immunogen Purified recombinant mouse GAD 2 (aa 3-96).
Clone 26H1
Isotype IgG3
Specificity Specific for GAD 2 / GAD 65
Purification purified IgG. Azide was added before lyophilization.
Alternative Name GAD 2 (GAD2 Antibody Abstract)
Background Synonyms: GAD 65
Application Notes WB: 1 : 1000 (AP staining)
ICC: 1 : 500
IHC: 1 : 200 up to 1 : 500
Comment

WB: This antibody is less sensitive than the rabbit antibody. ELISA: Suitable as capture antibody for sandwich-ELISA with ABIN1742419 as detector antibody (protocol for sandwich-ELISA).
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 100 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1?mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Storage -20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 3-96) antibody (ABIN1742417) dilution: 1 : 1000, sample: synaptic vesicle fraction of rat brain (LP2)
Immunocytochemistry (ICC) image for anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 3-96) antibody (ABIN1742417) Immunolabeling of cultured hippocampus neurons (dilution 1 : 500).
Immunohistochemistry (IHC) image for anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 3-96) antibody (ABIN1742417) Indirect immunohistochemistry of a PFA fixed mouse cerebellum section (dilution 1 : 5...
Product cited in: Pan, Marrs, Ryan: "Vesicular glutamate transporter 1 orchestrates recruitment of other synaptic vesicle cargo proteins during synaptic vesicle recycling." in: The Journal of biological chemistry, Vol. 290, Issue 37, pp. 22593-601, 2015 (PubMed).

Liu, Bickford, Held, Nyitrai, Südhof, Kaeser: "The active zone protein family ELKS supports Ca2+ influx at nerve terminals of inhibitory hippocampal neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 37, pp. 12289-303, 2014 (PubMed).

Coley, Ruffin, Moss, Hopfer, Boron: "Immunocytochemical identification of electroneutral Na?-coupled HCO?? transporters in freshly dissociated mouse medullary raphé neurons." in: Neuroscience, Vol. 246, pp. 451-67, 2013 (PubMed).