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  • 产品名称：anti-NCAM180(InternalRegion)antibody

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  • 产品厂商：SYSY
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简单介绍：
anti-NCAM180(InternalRegion)antibody
详情介绍：
Immunogen Detergent soluble membrane fraction of embryonic mouse brain. Epitope Binds to the intracellular part of NCAM 180.
Clone 481
Isotype IgG
Specificity Specific for NCAM 180.
Purification purified IgG
Background Synonyms: N-CAM 180
Application Notes WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 200 up to 1 : 500
Comment

This antibody lacks the Fc region and has to be used like a Fab fragment. Secondary reagents directed exclusively against the Fc region are not recommended.
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Storage -20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-NCAM 180 (Internal Region) antibody (ABIN1742446) dilution: 1 : 1000, sample: mouse brain homogenate
Immunocytochemistry (ICC) image for anti-NCAM 180 (Internal Region) antibody (ABIN1742446) Indirect immunostaining of rat hippocampus neurons with anti-NCAM 180 (dilution 1 : 2...
Product cited in: Schuster, Krug, Stalder, Hackel, Gerardy-Schahn, Schachner et al.: "Immunoelectron microscopic localization of the neural recognition molecules L1, NCAM, and its isoform NCAM180, the NCAM-associated polysialic acid, beta1 integrin and the extracellular matrix ..." in: Journal of neurobiology, Vol. 49, Issue 2, pp. 142-58, 2001 (PubMed).

Kramer, Hall, Bleistein, Schachner: "Developmentally regulated masking of an intracellular epitope of the 180 kDa isoform of the neural cell adhesion molecule NCAM." in: Journal of neuroscience research, Vol. 49, Issue 2, pp. 161-75, 1997 (PubMed).

Pollerberg, Sadoul, Goridis, Schachner: "Selective expression of the 180-kD component of the neural cell adhesion molecule N-CAM during development." in: The Journal of cell biology, Vol. 101, Issue 5 Pt 1, pp. 1921-9, 1985 (PubMed).