产品详情
所在位置: 首页> 产品目录> 一抗>
  • 产品名称:anti-SYT1antibody(SynaptotagminI)(AA1-8)

  • 产品型号:
  • 产品厂商:SYSY
  • 产品文档:
你添加了1件商品 查看购物车
简单介绍:
anti-SYT1antibody(SynaptotagminI)(AA1-8)
详情介绍:
Immunogen Synthetic peptide MVSASRPE (aa 1-8 in mouse synaptotagmin 1) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
Specificity Specific for synaptotagmin 1
Cross-Reactivity (Details) no cross-reactivity to synaptotagmin 2.
Purification Affinity purified with the immunogen. Guinea pig serum albumin was added for stabilization.
Alternative Name Synaptotagmin 1 (SYT1 Antibody Abstract)
Background Synonyms: p65
Research Area Neurology
Pathways Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
Application Notes WB: 1 : 2000 (AP staining)
IP: not tested yet
IHC: not tested yet
Comment

This antibody can be used for labeling of recycling synaptic vesicles by adding to living neurons or as a marker for exocytosis in isolated nerve terminals.

Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Add 1:1 (v/v) glycerol, then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Western Blotting (WB) image for anti-Synaptotagmin I (SYT1) (AA 1-8), (Lumenal Region) antibody (ABIN1742201) dilution: 1 : 2000, sample: crude synaptosomal fraction of rat brain (P2)
Immunocytochemistry (ICC) image for anti-Synaptotagmin I (SYT1) (AA 1-8), (Lumenal Region) antibody (ABIN1742201) Immunocytochemistry on PFA fixed cultured hippocampus neurons.
Product cited in: Shinoda, Ahmed, Ramachandran, Bharat, Brockelt, Altas, Dean: "BDNF enhances spontaneous and activity-dependent neurotransmitter release at excitatory terminals but not at inhibitory terminals in hippocampal neurons." in: Frontiers in synaptic neuroscience, Vol. 6, pp. 27, 2014 (PubMed).