anti-SV2Bantibody(SynapticVesicleGlycoprotein2B)(AA2-17) 抗体网

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产品名称：anti-SV2Bantibody(SynapticVesicleGlycoprotein2B)(AA2-17)
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产品厂商：SYSY
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简单介绍：

anti-SV2Bantibody(SynapticVesicleGlycoprotein2B)(AA2-17)

详情介绍：

Product Details anti-SV2B Antibody
Product Details anti-SV2B Antibody
Immunogen	Synthetic peptide DDYRYRDNYEGYAPND (aa 2-17 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Clone	246E8
Isotype	IgG2b
Specificity	Specific for SV2 B.
Purification	purified IgG

Target Details SV2B
Target Details SV2B
Alternative Name	SV2 B (SV2B Antibody Abstract)

Application Details
Application Details
Application Notes	WB: 1 : 1000 (AP staining) ICC: 1 : 100 up to 1 : 500 IHC: 1 : 200 IHC-P: 1 : 500
Comment	WB: SV2 aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples.
Restrictions	For Research Use only

Handling
Handling
Format	Lyophilized
Reconstitution	For reconstitution add 100 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer	PBS, 0.02% sodium azide (= 0.02 μg)
Preservative	Sodium azide
Precaution of Use	This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice	Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1?mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Storage	-20 °C
Storage Comment	Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Images
Images
Supplier Images	dilution: 1 : 1000, sample: crude synaptic vesicle fraction of rat brain (LP2) Indirect immunostaining of cultured hippocampus neurons (dilution 1 : 200). Indirect immunofluorescence labeling of a PFA fixed mouse cerebellum section (dilutio... Immunostaining of paraffin embedded sections from mouse brain (dilution 1 : 500). Imm... Images for product: anti-Synaptic Vesicle Glycoprotein 2B (SV2B) (AA 2-17) antibody dilution: 1 : 1000, sample: crude synaptic vesicle fraction of rat brain (LP2) Indirect immunostaining of cultured hippocampus neurons (dilution 1 : 200). Indirect immunofluorescence labeling of a PFA fixed mouse cerebellum section (dilution 1 : 200; red). Nuclei have been visualized by DAPI staining (blue). Immunostaining of paraffin embedded sections from mouse brain (dilution 1 : 500). Immunoreactivity was revealed using diaminobenzidine as chromagen. Nuclei were counterstained with haematoxylin (blue).

References for anti-SV2B antibody (ABIN1742257)
References for anti-SV2B antibody (ABIN1742257)
Product cited in:	Aroeira, Vaz, Sebasti?o, Valente: "BDNF modulates glycine uptake in hippocampal synaptosomes by decreasing membrane insertion of glycine transporter 2." in: Neurochemistry international, Vol. 99, pp. 94-102, 2016 (PubMed). Dagar, Nagar, Goel, Cherukuri, Dhingra: "Loss of photoreceptors results in upregulation of synaptic proteins in bipolar cells and amacrine cells." in: PLoS ONE, Vol. 9, Issue 3, pp. e90250, 2014 (PubMed).