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  • 产品名称：LiquidFast-RedSubstrateSystem

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  • 产品厂商：SpringBio
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简单介绍：
LiquidFast-RedSubstrateSystem
详情介绍：
Characteristics Liquid Fast Red can be used as chromogen for immunohistochemical staining. In the presence of alkaline phosphatase enzyme, Liquid Fast Red produces a red reaction product that can be seen using either brightfield or fluorescent microscopy.
Components Fast-Red Chromogen (25x) (55 mL), Naphthol Phosphate Substrate Buffer (1 L).
Comment

1. Use the working solution within 15 minutes of preparation or decreased sensitivity may result. 2. Do not put sodium azide in the buffer, it will prevent staining. 3. Precipitate is soluble in alcohol, thus aqueous counterstain and mounting medium should be used.
Protocol 1. To prepare working solution, add 40µl (one drop) Fast Red Chromogen to 1ml of Liquid Fast Red Substrate immediately before use.
2. Apply working solution to tissue section.
3. Incubate tissue section for 10-20 minutes.
4. Rinse in buffer. Apply counterstain according to manufacturer’s instructions.
5. Apply a sufficient volume of an aqueous mounting medium to cover the section.
Restrictions For Research Use only
Format Liquid
Concentration 25x
Precaution of Use 1. Take reasonable precautions when handling reagents. Wear appropriate Personal Protective Equipment.
2. Avoid contact of reagents with eyes, mucous membranes and skin. If reagents come into contact with sensitive areas, wash with copious amounts of water.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment The user must validate any other storage conditions. The reagents must be returned to the storage conditions identified above immediately after use. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.