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Characteristics
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High Binding Capactity: Binding of IgG ≥ 30 mg human or rabbit IgG/mL Protein G-Agarose.
Maximum Flow Rate* = 1800 cm/hr, minimum leaching of recombinant Protein G. Note * = the highest flow that beads withstand for 1 min, without collapsing and the pressure reaching 1 MPa.
Usage: Reusable for up to 10 times without significant loss of binding capacity
Applications: Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants. Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen. Hi-Bindtm Protein G-Agarose beads are specially prepared for high IgG binding by covalently coupling recombinant Protein G to 6 % cross-linked Agarose beads, the most popular resin for protein affinity purification methods. Protein G is a genetically engineered protein containing three IgG-binding regions of native Protein G. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein G to ensure the maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG & minimum leaching of recombinant Protein G than the other regular Protein-G Agarose on the market. The IgG binding capacity of Hi-Bindtm Protein G-Agarose is ≥ 30 mg of human or rabbit IgG per mL of wet beads. The Hi-Bindtm Protein G-Agarose beads are stable in chemical & physical properties with high flow rate, hydrophilicity & high gel strength.
Synonyms: Protein G-Agarose
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