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  • 产品名称：SDS-PAGEGelPreparationKit

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  • 产品厂商：Biospes
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简单介绍：
SDS-PAGEGelPreparationKit
详情介绍：
Characteristics SDS-PAGE Gel Preparation Kit contains various reagents for SDS-PAGE Gel Preparation, end user just need to prepare the experimental instruments and double distilled water separately. It can be used for preparing SDS-PAGE gel and native PAGE gel. This Kit is enough for preparing 30-50 pieces of PAGE gel with normal size. Kit Components Components
Sample Preparation
  1. Choose the proper concentration of gel according to the molecular weight of target protein. SDS-PAGE separation gel concentration (%) Molecular weight of target protein (kDa) 6% Gel 50-150 kDa 8% Gel 30-90 kDa 10% Gel 20-80 kDa 12% Gel 12-60 kDa 15% Gel 10-40 kDa 2. SDS-PAGE separation gel Component The volume of the components needed (ml) 6% Gel 5 10 15 20 30 50 Double distilled water 2.6 5.3 7.9 10.5 15.8 26.3 30% Acr-Bis (29:1) 1.0 2.0 3.0 4.0 6.0 10.0 Chongqing Biospes Co., Ltd Product Manual FOR RESEARCH USE ONLY, NOT FOR DIAGNOSTIC AND CLINICAL USE. Chongqing Biospes Co., Ltd Tel: +86-23-67567091 Fax: +86-23-67745923 Room 27-2, Building 7, No. 15, Paradise Walk, Jiangbei District, Chongqing, 400020, China www.biospes.com 2 1.5 M Tris, pH8.8 1.3 2.5 3.8 5.1 7.6 12.7 10% SDS 0.05 0.1 0.15 0.2 0.3 0.5 10% Ammonium persulfate 0.05 0.1 0.15 0.2 0.3 0.5 TEMED 0.004 0.008 0.012 0.016 0.024 0.04 Component The volume of the components needed (ml) 8% Gel 5 10 15 20 30 50 Double distilled water 2.3 4.6 6.9 9.2 13.8 23 30% Acr-Bis (29:1) 1.3 2.7 4.0 5.3 8.0 13.3 1.5 M Tris, pH8.8 1.3 2.5 3.8 5.1 7.6 12.7 10% SDS 0.05 0.1 0.15 0.2 0.3 0.5 10% Ammonium persulfate 0.05 0.1 0.15 0.2 0.3 0.5 TEMED 0.003 0.006 0.009 0.012 0.018 0.03 Component The volume of the components needed (ml) 10% Gel 5 10 15 20 30 50 Double distilled water 1.9 4.0 5.9 7.8 11.8 19.6 30% Acr-Bis (29:1) 1.7 3.3 5.0 6.7 10.0 16.7 1.5 M Tris, pH8.8 1.3 2.5 3.8 5.1 7.6 12.7 10% SDS 0.05 0.1 0.15 0.2 0.3 0.5 10% Ammonium persulfate 0.05 0.1 0.15 0.2 0.3 0.5 TEMED 0.002 0.004 0.006 0.008 0.012 0.02 Component The volume of the components needed (ml) 12% Gel 5 10 15 20 30 50 Double distilled water 1.6 3.3 4.9 6.5 9.8 16.3 30% Acr-Bis (29:1) 2.0 4.0 6.0 8.0 12.0 20.0 1.5 M Tris, pH8.8 1.3 2.5 3.8 5.1 7.6 12.7 10% SDS 0.05 0.1 0.15 0.2 0.3 0.5 10% Ammonium persulfate 0.05 0.1 0.15 0.2 0.3 0.5 TEMED 0.002 0.004 0.006 0.008 0.012 0.02 Chongqing Biospes Co., Ltd Product Manual FOR RESEARCH USE ONLY, NOT FOR DIAGNOSTIC AND CLINICAL USE. Chongqing Biospes Co., Ltd Tel: +86-23-67567091 Fax: +86-23-67745923 Room 27-2, Building 7, No. 15, Paradise Walk, Jiangbei District, Chongqing, 400020, China www.biospes.com 3 Component The volume of the components needed (ml) 15% Gel 5 10 15 20 30 50 Double distilled water 1.1 2.3 3.4 4.5 6.8 11.3 30% Acr-Bis (29:1) 2.5 5.0 7.5 10.0 15.0 25.0 1.5 M Tris, pH8.8 1.3 2.5 3.8 5.1 7.6 12.7 10% SDS 0.05 0.1 0.15 0.2 0.3 0.5 10% Ammonium persulfate 0.05 0.1 0.15 0.2 0.3 0.5 TEMED 0.002 0.004 0.006 0.008 0.012 0.02
Restrictions For Research Use only
Preservative Sodium azide, Thimerosal (Merthiolate)
Precaution of Use This product contains Sodium azide,Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C for 3 months, and at -20 °C for long term.