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  • 产品名称：alamarBlue®

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  • 产品厂商：Bio-Rad
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简单介绍：
alamarBlue®
详情介绍：
Purpose The cell proliferation assay reagent alamarBlue® is designed to provide a rapid and sensitive measure of cell proliferation and cytotoxicity in various human and animal cell lines, bacteria and fungi.
Characteristics alamarBlue® is an indicator dye, that incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and changes colour in response to the chemical reduction of growth medium, resulting from cell growth. The alamarBlue® cell proliferation assay reagent is designed to quantitatively measure the proliferation of various human and animal cell lines, bacteria and fungi.
Application Notes - Cell proliferation assays.
- The reagent can be used to establish proliferation or relative cytotoxicity in a cell proliferation assay.
- Baseline data for predicting the toxicity of related novel agents can be compared to baseline data with known in-vivo toxicity.
- alamarBlue® is for use between pH6.8 and pH7.4.
Protocol Cell proliferation assay.
- Growing cells cause a chemical reduction of alamarBlue®.
- Continued growth maintains a reduced environment. (fluorescent, red).
- Inhibit on of growth maintains an oxidized environment. (non-fluorescent, blue).
- Data may be collected using either fluorescence-based or absorbance-based instrumentation.
- Fluorescence is monitored at 530-560nm excitation wavelength and 590nm emission wavelength.
- Absorbance is monitored at 570nm and 600nm.
Restrictions For Research Use only
Format Liquid
Handling Advice This product is photosensitive and should be protected from light.
Storage 4 °C
Storage Comment This product may be stored for 12 months at room temperature. Store at 2-8°C to increase the shelf life to 20 months. This product should be stored undiluted.