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Application Notes
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Specific methodologies have not been tested using this product. Bovine serum albumin (BSA) and dry milk may contain IgG which reacts with anti-bovine IgG, anti-goat IgG, and anti-horse IgG, and anti-sheep IgG antibodies. Therefore, use of BSA and/or dry milk to block or dilute these antibodies and/or your primary antibody may significantly increase background and/or reduce secondary antibody titer. For pre-blocking, a 4-5 % normal serum from the same host species of the labeled secondary antibody is recommended.
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