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Immunogen
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Highly purified normal homogenous IgG2a(1b) isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Isotype
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IgG
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Specificity
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The reactivity of the antiserum is restricted to the Fc portion of the IgG2a molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against mouse serum of the allelic type Igh-1b, including C57Bl and SJL/J, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG2a(1b). No precipitation reaction is obtained with purified IgG2a(1a) (Balb/C, CBA, AKR and NZB), other IgG subclasses, IgA, IgG/Fab fragments or other serum proteinsPrecipitating polyclonal rabbit antiserum to mouse IgG2a(1b), allotype specific
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Characteristics
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Precipitating polyclonal rabbit antiserum to mouse IgG2a(1b), allotype specific
Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. Antibody titre: Precipitin titre 1:32 when tested against pooled normal Igh-1b allotype mouse serum in agar-block immun
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Purification
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Delipidated, heat inactivated, lyophilized, stable whole antiserum
Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
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