|
Characteristics
|
A423 cells were grown in Dulbecco's medium supplemented with 10 % fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0 % NP-40, 0.5 % sodium azide
|