产品详情
查看大图

  • 产品名称：A549WholeCellLysate

  • 产品型号：
  • 产品厂商：AvivaSysBio
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
A549WholeCellLysate
详情介绍：
Specificity Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents.
Characteristics Ready-to-use whole cell lysates are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. ﾠ All extracts are tested by SDS-PAGE using 4-20 % gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.
Application Notes The cells were grown in Dulbecco's medium supplemented with 10 % fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium cloride, 50 mM Tris HCI, pH 7.4, 1 mM EDTA, 1.0 % NP-40, 0.5 % sodium
Restrictions For Research Use only
Handling Advice Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Storage -20 °C/-80 °C
Storage Comment Store vial at -70 °C or COLDER. ﾠ For extended storage, aliquot contents to minimize freeze/thaw cycles. ﾠ Expiration date is three (3) months from date of opening if stored at -20 °C or (1) year from date of opening if stored at -70 °C.
Expiry Date 6 months