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  • 产品名称：Goatanti-Mouse(Murine)IgGAntibody(HRP)

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  • 产品厂商：BioLeaf
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简单介绍：
Goatanti-Mouse(Murine)IgGAntibody(HRP)
详情介绍：
Immunogen

Immunogen: Mouse IgG whole molecule
Isotype IgG
Characteristics HRP Conjugated Secondary Antibodies are ideal for western blot and are available in a variety of formats and conjugate types. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Purification HRP secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
Research Area Immunology, Secondary Antibodies
Application Notes

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Peroxidase Secondary antibody reagents are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.

Western Blot Dilution: 1:2,000 - 1:20,000

ELISA Dilution: 1:100,000 - 1:150,000
Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)
Concentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Gentamicin sulfate
Precaution of Use This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store secondary antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Anti-Mouse IgG HRP secondary antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiry Date 12 months