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  • 产品名称：Mouseanti-HumanIgA2(m)2Antibody

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  • 产品厂商：BioLeaf
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简单介绍：
Mouseanti-HumanIgA2(m)2Antibody
详情介绍：
Immunogen Highly purified monoclonal IgA2(m)2 isolated from human serum.
Clone NI 194-3 (A89-040)
Isotype IgG1 kappa
Specificity The reactivity of the antiserum is restricted to the IgA2(m)2 molecule as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immuno- precipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). Purified monoclonal mouse antibody to human IgA2(m)2, allotype specific
Characteristics Purified monoclonal mouse antibody to human IgA2(m)2, allotype specific
Purification Purified
Application Notes To identify the presence of IgA2 (m) 2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immuno-peroxidase and immunofluorescence staining of cytoplasmic IgA2 (m) 2, haemagglutination and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:200 upwards, in Western blotting from 1:500 upwards. These data should be interpreted as general recommendations only.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute the lyophilized ascites by adding 0. 5 mL sterile distilled water.
Concentration 0.4 mg/mL
Buffer Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2)
Handling Advice Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
Storage 4 °C/-20 °C
Storage Comment The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.