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  • 产品名称：Goatanti-Rat(Rattus)IgGAntibody(PE)

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  • 产品厂商：BioLeaf
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简单介绍：
Goatanti-Rat(Rattus)IgGAntibody(PE)
详情介绍：
Immunogen

Immunogen: Rat IgG F(ab')2 fragment
Isotype IgG
Fragment Fab fragment
Cross-Reactivity (Details) Pre-Adsorbed
Purification This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rat IgG, Rat IgG F(ab')2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.
Research Area Immunology, Secondary Antibodies
Application Notes

Flow Cytometry Dilution: 1:50 - 1:200

Application Note: Supplied in pH 7.6 buffer (10 mM Sodium Phosphate 250 mM NaCl) Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

IF Microscopy Dilution: 1:50 - 1:200
Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)
Concentration 0.5 mg/mL
Buffer

Buffer: See application note.

Stabilizer: 15 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.
Expiry Date 12 months