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  • 产品名称：Goatanti-HumanIgA,IgG,IgMAntibody(Biotin)

  • 产品型号：
  • 产品厂商：BioLeaf
  • 产品文档：
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简单介绍：
Goatanti-HumanIgA,IgG,IgMAntibody(Biotin)
详情介绍：
Immunogen

Immunogen: Human IgG, IgA and IgM whole molecules
Isotype IgG
Fragment Fab fragment
Cross-Reactivity (Details) Pre-Adsorbed
Characteristics Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Purification This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Goat Serum. No reaction was observed against Mouse Serum proteins. This product is suitable for the detection of all Human immuno-globulin classes, isotypes and chain combinations.
Alternative Name IgG IgA IgM
Application Notes

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: This product has been assayed against 1.0 μg of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:20,000 - 1:100,000
Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)
Concentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiry Date 12 months