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  • 产品名称:Rabbitanti-GoatIgM(Heavy&LightChain)Antibody

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  • 产品厂商:BioLeaf
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简单介绍:
Rabbitanti-GoatIgM(Heavy&LightChain)Antibody
详情介绍:
Immunogen Purified normal IgM isolated from pooled goat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Characteristics Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit serum.

Physical form: Delipidated, heat inactivated lyophilized stable whole serum.
Purification Antisera
Adsorption: No adsorption required.
Background In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgM and IgG antibodies, as secondary antibody to precipitate the immunoglobulin in normal goat serum, to prepare an immunoadsorbent for the production of Ig-free goat serum, plasma or non-Ig preparations.
Directions for use: In immunoelectrophoresis use 2 µl or equivalent against 120 µl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Research Area Secondary Antibodies
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute the lyophilized ascites by adding 0.5 mL sterile distilled water.
Buffer No foreign protein added.
Preservative Without preservative
Handling Advice Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation. This will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
Storage 4 °C/-20 °C
Storage Comment The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.