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  • 产品名称：Goatanti-Mouse(Murine)IgG2bAntibody

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  • 产品厂商：BioLeaf
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简单介绍：
Goatanti-Mouse(Murine)IgG2bAntibody
详情介绍：
Immunogen Pools of purified homogenous IgG2b isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Isotype IgG
Specificity The reactivity of the antiserum is directed to the subclass IgG2b.
Characteristics Total protein and IgG concentration in the antiserum are comparable to those of pooled normal goat serum. Antibody titre: Precipitin titre not les than 1:32 when tested against pooled normal mouse serum in agar block immunodiffu
Purification Delipidated, heat inactivated, lyophilized, stable whole antiserum
Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Research Area Secondary Antibodies
Application Notes In immunoelectrophoresis use 2 µL or equivalent against 120 µL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 µL antiserum in a 3 mm diameter centre well and 2 µL serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
Buffer No foreign proteins added.
Preservative Without preservative
Handling Advice Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 °C, not ref rozen, and preferably used the same day.
Storage 4 °C/-20 °C
Storage Comment The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.