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  • 产品名称：Goatanti-FerretIgAAntibody(Biotin)

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  • 产品厂商：BioLeaf
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简单介绍：
Goatanti-FerretIgAAntibody(Biotin)
详情介绍：
Immunogen

Immunogen: Ferret IgA alpha heavy chain
Isotype IgG
Fragment Fab fragment
Characteristics Ferret IgA whole molecule antibody generated in goat detects specifically ferret IgA whole molecule. Anti-Human IgA is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays
Purification Anti-FERRET IgA (alpha chain) (GOAT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Ferret IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Ferret IgA and Ferret Serum. No reaction was observed against Ferret IgM or Ferret IgG.
Research Area Immunology, Secondary Antibodies
Application Notes

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-FERRET IgA (alpha chain) (GOAT) Antibody has been assayed against 1.0 μg of Ferret IgA in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:20,000 is suggested for this product.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:20,000 - 1:100,000
Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)
Concentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiry Date 12 months