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  • 产品名称：Mouseanti-HumanIgA2Antibody

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  • 产品厂商：BioLeaf
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简单介绍：
Mouseanti-HumanIgA2Antibody
详情介绍：
Immunogen Highly purified monoclonal IgA2 isolated from pooled human serum
Clone NI 512 (A89-038)
Isotype IgG1 kappa
Specificity The reactivity of the antiserum is restricted to a subclass specific determinant on the IgA2 molecule as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). Monoclonal mouse antiserum to human IgA2
Characteristics Monoclonal mouse antiserum to human IgA2
Purification Delipidated, heat inactivated, lyophilized, stable whole ascites
Research Area Secondary Antibodies
Application Notes To identify the presence of IgA2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immuno-peroxidase and immunofluorescence staining of cytoplasmic IgA2, haemagglutination and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1000 upwards. These data should be interpreted as general recommendations only.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute the lyophilized ascites by adding 0. 5 mL sterile distilled water.
Concentration 1 mg/mL
Buffer No foreign proteins added.
Handling Advice Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
Storage 4 °C/-20 °C
Storage Comment The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.