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Immunogen
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Purified normal IgG, including all known subclasses, isolated from pooled mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Isotype
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IgG
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Specificity
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The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. The antiserum contains antibodies to subclass-specific determinants as well as to determinants shared by two or more subclasses of IgG. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal mouse plasma, serum, and immunoglobulin fractions,
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Characteristics
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Precipitating polyclonal rabbit antiserum to mouse IgG, Fc specific
Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit serum. Antibody titre: Precipitin titre not less than 1:64 when tested against normal mouse serum in agar block titration.
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Purification
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Delipidated, heat inactivated lyophilized stable whole serum
Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the elimination of antibodies to the common Ig/Fab part. The use of insolubilized adsorption antigens prevents the presence of foreign protein or immune complexes in the antiserum.
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