Immunogen
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Pools of purified normal IgG2 isolated from pooled guinea pig serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Isotype
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IgG
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Specificity
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The reactivity of the antiserum is directed to the Fc subunit of subclass IgG2.
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Characteristics
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Precipitating polyclonal goat antiserum to guinea pig IgG2, subclass specific Total protein and IgG concentration in the antiserum are comparable to those of pooled normal goat serum. Antibody titre: Precipitin titre not less then 1:32 when tested against pooled normal guinea pig serum in agar block immun
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Purification
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Delipidated, heat inactivated, lyophilized whole antiserum. Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
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