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  • 产品名称：Sheepanti-Horse(Equine)IgG(Heavy&LightChain)Antibody

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  • 产品厂商：BioLeaf
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简单介绍：
Sheepanti-Horse(Equine)IgG(Heavy&LightChain)Antibody
详情介绍：
Immunogen Purified normal IgG isolated from pooled horse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Isotype IgG
Specificity The reactivity of the antiserum is directed to the Fc and Fab subunits of both subclasses IgG1 and IgG2. Reactivity with other subclasses of horse IgG has not been tested. The antiserum reacts with both Fc and Fab portions of polyclonal IgG. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. In immunoelectrophoresis against horse serum usually a single characteristic precipitin line is observed, representing primarily IgG2. If the level of IgG1, also known as IgG(T), in the serum sample approaches the level of IgG2, it may become visible as a second precipitin line.
Characteristics Total protein and IgG concentration in the antiserum are comparable to those of pooled sheep serum. Antibody titre: Precipitin titre not less than 1:32 when tested against normal horse serum in agar block titration.
Purification Delipidated, heat inactivated lyophilized stable whole serum
No adsorption required.
Research Area Immunology, Secondary Antibodies
Application Notes In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG, as secondary antibody to precipitate the immunoglobulin in normal horse serum, to prepare an immunoadsorbent for the production of Ig-free horse serum, plasma or non-Ig preparations. It has not been tested for use in nephelometry, ELISA or immunohistology, but this does not exclude such use if proper controls are included.
Restrictions For Research Use only
Format Lyophilized
Reconstitution It is reconstituted by adding 1 mL sterile distilled water.
Buffer No foreign proteins added.
Preservative Without preservative
Handling Advice Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same da y. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Storage 4 °C/-20 °C
Storage Comment The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.