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  • 产品名称：Goatanti-Rat(Rattus)IgE(FcRegion)Antibody

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  • 产品厂商：BioLeaf
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简单介绍：
Goatanti-Rat(Rattus)IgE(FcRegion)Antibody
详情介绍：
Immunogen Purified homogenous IgE isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Isotype IgG
Specificity The reactivity of the antiserum is directed to the Fc subunits of the IgE molecule which represents strict isotype (class) specificity. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against appropriate concentrations of IgE, a single precipitin line is obtained which shows a reaction of full identity with the precipitin line obtained with the used immunogen.
Characteristics Purified IgG fraction of polyclonal goat antiserum to rat IgE, Fc specific
Purification Purified
Research Area Immunology, Secondary Antibodies
Application Notes Performance testing Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of rat cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays. As unlabelled secondary antibody for indirect detection of IgE in rat cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods, for the production of immunoconjugates with a selected marker.
Product can be used to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier, as catching or detecting antibody reagent in non-isotopic assay methodology (e. g. ELISA) to identify and measure IgE in rat serum or other body fluid. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography. Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibody activity reacting with other components of the immunoglobulin system and to any other serum protein. Special attention is given to the elimination of antibodies to the common Fab portion of immunoglobulins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Restrictions For Research Use only
Format Lyophilized
Reconstitution It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
Concentration 10 mg/mL
Buffer lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
Preservative Without preservative
Handling Advice Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2).
Repeated thawing and freezing should be avoided.
Working dilutions should be stored at +0 °C , not refrozen, and preferably used the same day.
If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Storage (shelf life): Lyophilized at +0 °C (at least 10 years). Reconstituted at or below -20 °C (3-5 years). Reconstituted at +0 °C (7 days).
Storage 4 °C
Storage Comment prolonged storage at or below -20 °C.
Expiry Date 36 months