Hela (Lane 1) and NIH 3T3 (Lane 2) Whole cell lysates were run on a 4-20% gel, transferred to nitrocellulose under standard conditions, and incubated with beta Actin antibody at a dilution of 1:2000 (ON 4 deg C). Goat anti Rabbit IgG (H + L) (HRP) was used to for detection.