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  • 产品名称：Rabbitanti-GoatIgG(Heavy&LightChain)Antibody(HRP)

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  • 产品厂商：BioLeaf
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简单介绍：
Rabbitanti-GoatIgG(Heavy&LightChain)Antibody(HRP)
详情介绍：
Immunogen Anti-Goat IgG whole molecule was produced by repeated immunization with Goat IgG whole molecule in rabbit.
Immunogen Type: Native Protein
Fragment F(ab')2 fragement
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG and Goat Serum.
Characteristics F(ab')2 anti-Goat IgG (H&L) peroxidase conjugated antibody generated in rabbit detects specifically goat IgG (H&L). This secondary conjugated antibody anti-goat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Purification This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Background Synonyms: F(ab')2 Goat IgG (H&L) Antibody, Rb-a-F(ab')2 Goat IgG(H&L) Peroxidase conjugated, F(ab')2 Goat IgG(H&L) antibody in rabbit, F(ab')2 Goat IgG (H&L) Peroxidase conjugated Secondary Antibody.
Research Area Immunology, Secondary Antibodies
Application Notes This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 is suggested for this product.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Gentamicin sulfate
Handling Advice Do NOT add Sodium Azide!
Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
Expiry Date 12 months