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  • 产品名称：Mouseanti-HumanIgG3Antibody(FITC)

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  • 产品厂商：BioLeaf
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简单介绍：
Mouseanti-HumanIgG3Antibody(FITC)
详情介绍：
Immunogen Highly purified monoclonal IgG3 isolated from human serum.
Clone NI 330 (HP 6050)
Isotype IgG1 kappa
Specificity The reactivity of the antiserum is restricted to a subclass specific determinant on the F(ab')2 part of the IgG3 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunoperoxidase staining of cytoplasmic Ig. Fluorescein isothiocyanate-conjugated purified monoclonal mouse antibody to human IgG3, subclass specific
Characteristics Fluorescein isothiocyanate-conjugated purified monoclonal mouse antibody to human IgG3, subclass specific
Fluorochrome/IgG protein molar ratio (F/P) approximately 1.0.
Purification Purified
Research Area Secondary Antibodies
Application Notes To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:50, in ELISA from 1:200 upwards, in Western blotting from 1:400 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.
Comment

Excitation/Emission wavelength: 494 nm/514 nm
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.
Concentration 0.4 mg/mL
Buffer Purified monoclonal mouse IgG1 kappa conjugated with FITC, lyophilized from a solution in phosphate buffered saline (pH 7.2).
Preservative Without preservative
Handling Advice Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
Storage 4 °C/-20 °C
Storage Comment The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.