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  • 产品名称：Mouseanti-HumanIgA2Antibody(TRITC)

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  • 产品厂商：BioLeaf
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简单介绍：
Mouseanti-HumanIgA2Antibody(TRITC)
详情介绍：
Immunogen Highly purified monoclonal IgA2 isolated from human serum.
Clone NI 512 (A89-038)
Isotype IgG1 kappa
Specificity The reactivity of the antiserum is restricted to an subclass specific determinant on IgA2 as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglu-tination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). Tetramethylrhodamine isothiocyanate-conjugated purified monoclonal mouse antibody to human IgA2, subclass specific
Characteristics Tetramethylrhodamine isothiocyanate-conjugated purified monoclonal mouse antibody to human IgA2, subclass specific
Fluorochrome/IgG protein molar ratio (F/P) approximately 2.5.
Purification Purified
Research Area Secondary Antibodies
Application Notes To identify the presence of IgA2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as immunofluorescence staining of cytoplasmic IgA2, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC.
The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 to 1:100, in Western blotting from 1:200 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.
Concentration 0.4 mg/mL
Buffer Purified monoclonal mouse IgG1 kappa conjugated with TRITC, lyophilized from a solution in phosphate buffered saline (pH 7.2).
Preservative Without preservative
Handling Advice Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
Storage 4 °C/-20 °C
Storage Comment The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.