产品详情
  • 产品名称:HumanTumorTissueBlotIV

  • 产品型号:
  • 产品厂商:ProSci-Inc
  • 产品文档:
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简单介绍:
HumanTumorTissueBlotIV
详情介绍:
Characteristics The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different human tumor tissue lysates with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20 % SDS-PAGE gel and transferred onto nitrocellulose membrane. The human tumor tissues included in the blot are kidney, liver, lymphoma, Non-Hodgkin's lymphoma, spleen, thymoma, thyroid, and tonsil.
Sample diagnosis: Tumor
Samples loaded: Lanes: 1: protein markers, 2: kidney, 3: liver, 4: lymphoma, 5: Non-Hodgkins lymphoma, 6: spleen, 7: thymoma, 8: thyroid, and 9: tonsil.
Quantity loaded per lane: 15 μg
Gel percentage: Gradient 4-20 %
Application Notes For Western Blot, the blot can be blocked with 1 % BSA or 5 % nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
Restrictions For Research Use only
Handling Advice The blot can be reused after stripping.
Storage 4 °C
Storage Comment Store at 4 °C.