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Characteristics
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The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20 % SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are appendix, kidney, liver, lymph node, peripheral blood leukocyte, spleen, thymus, tonsil, and thyroid.
Sample diagnosis: Normal
Samples loaded: Lanes: 1: protein markers, 2: appendix, 3: kidney, 4: liver, 5: lymph node, 6: peripheral blood leukocyte, 7: spleen, 8: thymus, 9: tonsil, and 10: thyroid
Quantity loaded per lane: 15 μg
Gel percentage: Gradient 4-20 %
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