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  • 产品名称：VascularEndothelialCellGrowthFactor121(VEGF121)(AA207-327)Peptide

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  • 产品厂商：BioLeaf
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简单介绍：
VascularEndothelialCellGrowthFactor121(VEGF121)(AA207-327)Peptide
详情介绍：
Sequence MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQEKCDKP RR
Characteristics Purified recombinant Human VEGF121 protein
Expression System: E.coli
Bioactivity: The ED50 for this effect is
Molecular weight on SDS-PAGE will appear higher.
Purity > 95 % pure
Background Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant VEGF121 protein was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Alternative Names: VEGF-A protein, Vascular endothelial growth factor A protein, VPF protein, VEGF protein, VEGF A., Vascular permeability factor protein, Vascular endothelial growth factor A isoform f protein, Vascular endothelial growth factor A isoform f MVCD1 protein
Molecular Weight 14.2 kDa (122 AA)
Research Area Cytokines, Growth Factors
Application Notes VEGF protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
Assay Procedure

Cell Line: NIH-3T3 (Mus mosculus/ embryo) Maintenance Condition: RPMI 1640 containing 10 % FBS Assay Medium: serum free RPMI 1640 Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100 µL) Serum Free Starvation: 24 h with RPMI1640 Incubation Time: 24 hr (after sample treatment) Concentration Range: 100 pg/mL - 10 µg/mL Detection method: BrdU assay
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 10 % glycerol.
Handling Advice Avoid repeated freeze/thaw cycles.
Storage RT/-20 °C
Storage Comment Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
Supplier Images
SDS-PAGE (SDS) image for Vascular Endothelial Cell Growth Factor 121 (VEGF121) (AA 207-327) peptide (ABIN935025) Figure annotation denotes ug of protein loaded and % gel used.