Lane 1: Rabbit IgG F(ab'')2 . Load: 100 ng per lane. Primary antibody: F(ab'')2 Rabbit IgG F(ab'')2 antibody Pre-Adsorbed at 1:1000 ON at 4 deg C. Secondary antibody: Dylight (TM) 800 Donkey anti-goat at 1:20, 000 for 30 min at RT. Block for 30 min at RT.