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  • 产品名称：Goatanti-Mouse(Murine)IgG(FcRegion)Antibody(HRP)

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  • 产品厂商：BioLeaf
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简单介绍：
Goatanti-Mouse(Murine)IgG(FcRegion)Antibody(HRP)
详情介绍：
Immunogen Mouse IgG F(c) fragment
Fragment F(ab')2 fragment
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum.
Purification This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Background Synonyms: F(ab')2 Mouse IgG F(c) Antibody, Goat-a-Mouse F(ab')2 IgG F(c) Peroxidase conjugated, Mouse F(ab')2 IgG F(c) Peroxidase Antibody in goat, Goat F(ab')2 peroxidase conjugated Secondary Antibody.
Research Area Immunology, Secondary Antibodies
Application Notes F(ab') 2 Anti-Mouse IgG F(c) Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10^6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Gentamicin sulfate
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Storage -20 °C
Storage Comment Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.
Expiry Date 12 months