Western Blot of Goat anti-F(ab')2 Rat IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed Lane 1: Rat IgG Load: 50 ng per lane Secondary antibody: F(ab')2 Rat IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed at 1:1,000 for 60 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa